sup 60 Co. gamma. -rays induce predominantly C/G to G/C transversions in double-stranded M13 DNA
- Physics Laboratory of the Free University, Amsterdam (Netherlands)
- State Univ. of Leiden (Netherlands)
- Physics Laboratory of the Free University, Amsterdam (Netherlands) Free University Hospital, Amsterdam (Netherlands)
Upon irradiation with gamma rays of an oxygenated aqueous solution of double-stranded M13 DNA, a very specific mutation spectrum was found with respect to both the type and the positions in the DNA sequence. Of the 23 mutations, which were sequenced, 16 represent a C/G to G/C transversion. A C/G to T/A transition was found once and a G/C to T/A transversion twice. The remaining 4 mutations are frameshifts, 2 are identical and formed by the insertion of a G/C basepair; the other 2 mutations are due to a duplication of 10 basepairs situated at different positions but with a remarkable homology in base sequence. Fourteen mutations, including the 2 duplications are found in the neighborhood of a TGCT/ACGA sequence.
- OSTI ID:
- 6945152
- Journal Information:
- Nucleic Acids Research; (UK), Vol. 16:16; ISSN 0305-1048
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
GENE MUTATIONS
RADIOINDUCTION
AQUEOUS SOLUTIONS
COBALT 60
DNA BASE TRANSITIONS
GAMMA RADIATION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
COBALT ISOTOPES
DISPERSIONS
ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
MIXTURES
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOLUTIONS
YEARS LIVING RADIOISOT
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture