Parasitophorous vacuole membrane of Plasmodium knowlesi

Conference · Thu May 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6943182

Nillni, E A; Wallach, D F.H.

The authors have evaluated the occurrence of host cell membrane protein and parasite protein in the vacuolar membrane (VM) of isolated parasites. Parasites were labeled by incorporation of (/sup 35/S)methionine and by lactoperoxidase-catalyzed /sup 125/I iodination. Of the two prominent /sup 125/I-labeled components, one, not detected by metabolic labeling corresponded in M/sub r/ to erythrocyte band 3 (90 kDa). Trypsinization of radioiodinated parasites for 5' or 20' yield a 35 kDa fragment, not seen in untreated samples and compatible with the trypsin degradation of band 3 from the cytoplasmic side. Tryptic peptide maps of the 35 kDa revealed a very acidic peptide corresponding to the highly anionic tryptic peptide of band 3 showed by others. The second prominent /sup 125/I-labeled VM protein had an M/sub r/ 74,000 corresponding to a protein metabolically labeled with (/sup 35/S)methionine, suggesting it is inserted into the VM by the parasites. Several less prominent proteins labeling with both (/sup 35/S)methionine and /sup 125/I were also detected (140 kDa, 55 kDa, 45 kDa). A faint /sup 125/I-labeled triple (220-230 kDa) is compatible with a trace amounts of spectrin, usually a prominent component of red cell membrane. The results indicate that host cell band 3 is a prominent component of the VM, but that this membrane also contains several parasite-synthesized proteins.

• Research Organization: Tufts-New England Medical Center, Boston, MA
• OSTI ID: 6943182
• Report Number(s): CONF-8606151-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANES
METHIONINE
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PLASMODIUM
PROTEINS
PROTOZOA
RADIOISOTOPES
SPOROZOA
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES