/sup 125/I-insulin metabolism by rat liver cells: preferential binding and degradation of insulin labeled at TyrA14

Journal Article · · Drug Metab. Dispos.; (United States)

OSTI ID:6943084

Misbin, R I; Buynitzky, S J; Almira, E C

The binding and degradation of highly purified A14-(/sup 125/I)monoiodoinsulin and A19-(/sup 125/C)monoiodoinsulin were compared in isolated rat hepatocytes. A14-/sup 125/I-insulin was bound more rapidly and was degraded more rapidly than was A19-/sup 125/I-insulin. The difference in the rate of degradation between the two preparations was apparent when either tricholoroacetic acid precipitation or binding to specific insulin receptors on cultured human lymphocytes was used for assay. These results provide evidence that binding to its receptor is the first step in insulin degradation by liver cells.

• Research Organization: Endocrinology and Metabolism, Department of Medicine, University of Florida
• OSTI ID: 6943084
• Journal Information: Drug Metab. Dispos.; (United States), Journal Name: Drug Metab. Dispos.; (United States) Vol. 10:2; ISSN DMDSA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIVER
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HORMONES
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
VERTEBRATES