Effect of glycerol on 3-hydroxybutyrate utilization by developing rat brain

Journal Article · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6942908

McKenna, M C; Bezold, L I; Tildon, J T

Earlier studies by the group showed that administration of glycerol to rat pups decreased plasma 3-hydroxybutyrate (3-HOB) levels. Since 3-HOB is an important substrate used for energy and lipid biosynthesis during early brain development they determined the effect of glycerol on the rate of incorporation of 3-HOB into lipids and proteins by developing rat brain. Eleven day old rat pups were made hyperglycerolemic by injection of glycerol in saline (controls were injected with saline), and given a concurrent subscapular injection of 2..mu..Ci of 3-hydroxy-3-/sup 14/-butyrate (/sup 14/C-HOB), maintained at 38/sup 0/C, and sacrificed after 60 min. Trunk blood was collected for determination of total radioactivity, 3-HOB and glycerol levels. Plasma 3-HOB levels were significantly lower (p<0.01) in glycerol treated rat pups than controls, 557 +/- 57 ..mu..M (mean +/- SEM) and 2240 +/- 450 ..mu..M, respectively. The rates of incorporation of /sup 14/C-HOB into brain lipids were 50 +/- 46 nmol/hr/mg brain protein for glycerol treated rats and 2590 +/- 652 nmol/hr/mg brain protein for controls, p<0.02. The rate of incorporation of /sup 14/C-HOB into protein was also decreased (p<0.02) in glycerol-treated rats; with 134 +/- 10 nmol incorp/hr/mg prot in glycerol treated rats and 884 +/- 217 nmol incorp/hr/mg brain protein (mean +/- SEM) for controls. These data show that elevated levels of plasma glycerol decrease circulating 3-HOB levels and thereby adversely affect the utilization of 3-HOB by developing brain.

• Research Organization: Univ. of Maryland School of Medicine, Baltimore
• OSTI ID: 6942908
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
BRAIN
BUTYRIC ACID
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
GLYCEROL
HYDROXY COMPOUNDS
HYPERGLYCEMIA
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MATERIALS
METABOLISM
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROTEINS
RATS
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES