Disintegration of nucleoskeletal elements by metrizamide/2 M salt isopyknic centrifugation
Supramolecular structures that remain bound to chromosomal DNA under high salt conditions are believed to anchor DNA in the interphase nuclear skeleton. In order to identify these anchorage structures, the non-DNA materials that remain firmly bound to chromosomal DNA under conditions that disintegrate the high salt-stable architecture of nuclei were investigated. Nuclei of Ehrlich ascites cells were histone-depleted by treatment with 2 M salt. The residual halo structures were gently sheared and subjected to metrizamide isophyknic centrifugation in the presence of 2 M salt. By this combined treatment the high slat stable nuclear skeleton becomes disintegrated and three main fractions are resolved. The results indicate that nascent RNP is more tightly bound to chromosomal DNA than other components that may be involved in nuclear skeletons. This suggests that transcription complexes represent at least one type of anchorage structure of DNA, which is consistent with results indicating that nascent RNA and actively transcribed DNA sequences are preferentially retained in high-salt-treated nuclei.
- Research Organization:
- Institute of Cell and Tumor Biology, Heidelberg, West Germany
- OSTI ID:
- 6942660
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 167:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ASCITES TUMOR CELLS
CENTRIFUGATION
DNA
COMPLEXES
METRIZAMIDE
BIOLOGICAL EFFECTS
PROTEINS
CARBON 14 COMPOUNDS
CELL NUCLEI
CHROMOSOMES
ELECTRON MICROSCOPY
IODINE 125
LABELLED COMPOUNDS
METHIONINE
MONOCLONAL ANTIBODIES
SALTS
SULFUR 35
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URIDINE
AMIDES
AMINO ACIDS
ANIMAL CELLS
ANTIBODIES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONTRAST MEDIA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROSCOPY
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SEPARATION PROCESSES
SULFUR ISOTOPES
TUMOR CELLS
URACILS
550301* - Cytology- Tracer Techniques