Mercury-binding proteins in the slipper limpet, Crepidula fornicata, exposed to increased soluble mercury

Journal Article · Sun Mar 01 00:00:00 EST 1987 · Estuaries; (USA)

DOI:https://doi.org/10.2307/1352028· OSTI ID:6942576

Harrison, F L; Watness, K ^[1]; Nelson, D A; Miller, J E; Calabrese, A ^[2]

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1. Lawrence Livermore National Lab., CA (USA)
2. Milford Lab., CT (USA)

Crepidula fornicata were held in a flow-through bioassay system and exposed to sand-filtered seawater to which no soluble mercury (control) was added or to which either 5, 25, or 50 {mu}g l{sup {minus}1} soluble Hg was added. At specific intervals during the 16-week experiment, a group of limpets was removed from each tank; one subgroup was exposed for 48 h to high concentrations of Hg, and another was analyzed for Hg-binding proteins by gel-permeation chromatography and spectrometry. Mortality from exposure to Hg in the 48-Hg acute toxicity tests was related to concentrations of Hg experienced both during the long-term exposure period and the 48-h exposure period. Chronic exposure to low levels of Hg resulted in increased amounts of total Hg in the whole body and in the low-molecular-weight Hg-binding proteins. No evidence was found for increased tolerance of Hg with preexposure.

• OSTI ID: 6942576
• Journal Information: Estuaries; (USA), Vol. 10:1; ISSN 0160-8347
• Country of Publication: United States
• Language: English

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