Passive transfer of leishmania lipopolysaccharide confers parasite survival in macrophages
Infection of macrophages by the intracellular protozoan parasite Leishmania involves specific attachment to the host membrane, followed by phagocytosis and intracellular survival and growth. Two parasite molecules have been implicated in the attachment event: Leishmania lipopolysaccharide (L-LPS) and a glycoprotein (gp63). This study was designed to clarify the role of L-LPS in infection and the stage in the process of infection at which it operates. The authors have recently identified a Leishmania major strain (LRC-L119) which lacks the L-LPS molecule and is not infective for hamsters or mice. This parasite was isolated from a gerbil in Kenya and was identified phenotypically as L. major by isoenzyme and fatty acid analysis. In this study they have confirmed at the genotype level that LRC-L119 is L. major by analyzing and comparing the organization of cloned DNA sequences in the genome of different strains of L. major. Here they show that LRC-L119 promastigotes are phagocytosed rapidly by macrophages in vitro, but in contrast to virulent strains of L. major, they are then killed over a period of 18 hr. In addition, they show that transfer of purified L-LPS from a virulent clone of L. major (V121) into LRC-L119 promastigotes confers on them the ability to survive in macrophages in vitro.
- Research Organization:
- Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
- OSTI ID:
- 6942081
- Journal Information:
- J. Immunol.; (United States), Vol. 137:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LIPOPOLYSACCHARIDES
BIOCHEMISTRY
PARASITIC DISEASES
IMMUNOLOGY
PROTOZOA
RADIOIMMUNOASSAY
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
GLUCOSE
IODINE 125
MACROPHAGES
PARASITES
PHAGOCYTOSIS
TRITIUM COMPOUNDS
ALDEHYDES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CHEMISTRY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
HEXOSES
IMMUNOASSAY
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIPIDS
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550701 - Microbiology- Tracer Techniques