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Photoaffinity labeling of opioid receptor with morphine-7,8-oxide (morphine epoxide)

Journal Article · · J. Pharmacol. Methods; (United States)
OSTI ID:6941363
; ; ;

The opioid receptor mediating inhibitory action of morphine in the electrically stimulated guinea pig ileum was irreversibly photoinactivated by morphine epoxide (3 X 10(-6) M). Morphine epoxide (up to 3 X 10(-5) M) did not influence the responses of rat vas deferens (epsilon-receptor) or rabbit vas deferens (kappa-receptor) to electrical stimulation. Effective concentrations of morphine epoxide were much lower in the guinea pig ileum (mu-receptor) than in the mouse vas deference (delta-receptor). The inhibitory action of (Met)-enkephalin on the twitch responses of the rat vas deferens and mouse vas deferens to electrical stimulation were not influenced after irradiation in the presence of morphine epoxide (3 X 10(-6) M). Therefore, morphine epoxide is probably a useful probe for photoaffinity labeling of the mu-receptor in vitro.

Research Organization:
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Chiba, Japan
OSTI ID:
6941363
Journal Information:
J. Pharmacol. Methods; (United States), Journal Name: J. Pharmacol. Methods; (United States) Vol. 7:3; ISSN JPMED
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALOIDS
ANALGESICS
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMISTRY
DIGESTIVE SYSTEM
DRUGS
EPOXIDES
EXTERNAL IRRADIATION
GASTROINTESTINAL TRACT
GUINEA PIGS
INTESTINES
IRRADIATION
MALE GENITALS
MAMMALS
MORPHINE
NARCOTICS
OPIUM
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHARMACOLOGY
PHOTOCHEMISTRY
RADIOSENSITIVITY EFFECTS
RECEPTORS
RODENTS
SMALL INTESTINE
VERTEBRATES