Effects of ancillary ligands on selectivity of protein labeling with platinum(II) chloro complexes
Potassium (2,6-pyridinedicarboxylato)chloroplatinate(II) was synthesized. The molecular structure of the complex in (n-Bu){sub 4}N(Pt(dipic)Cl){center dot}0.5H{sub 2}O was determined by x-ray crystallography. The (Pt(dipic)Cl){sup {minus}} is essentially planar and contains a Pt(II) atom, a tridentate dipicolinate dianion ligand, and a unidentate Cl{sup {minus}} ligand. The bis(bidentate) complex trans-(Pt(dipic){sub 2}){sup 2{minus}} was also observed by {sup 1}H NMR. A red gel-like substance was observed when the yellow aqueous solution of K(Pt(dipic)Cl) was cooled or concentrated. The K(Pt(dipic)Cl) molecules form stacks in the solid state and gel-like substance but remain monomeric over a wide range of concentrations and temperatures. The reactivity and selectivity of(Pt(dipic)Cl){sup {minus}} toward cytochromes c from horse and tuna were studied. The new transition-metal reagent is specific for methionine residues. Di(2-pyridyl-{beta}-ethyl)sulfidochloroplatinum(II) chloride dihydrate was also synthesized. This complex labels histidine and methionine residues in cytochrome c. The ancillary ligands in these platinum(II) complexes clearly determine the selectivity of protein labeling. 106 refs., 10 figs., 11 tabs.
- Research Organization:
- Ames Lab., IA (USA)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6941351
- Report Number(s):
- IS-T-1434; ON: DE90011805
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400201 -- Chemical & Physicochemical Properties
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
AZOLES
BOND LENGTHS
CARBOXYLIC ACIDS
CHEMICAL BONDS
CHEMICAL PREPARATION
CHEMICAL SHIFT
CHLORINE COMPOUNDS
COHERENT SCATTERING
COMPLEXES
CRYSTALLOGRAPHY
CYTOCHROMES
DIFFRACTION
DIMENSIONS
DRUGS
FISHES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HORSES
HYDROGEN 1
HYDROGEN ISOTOPES
IMIDAZOLES
ISOTOPES
LABELLING
LENGTH
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PLATINUM COMPLEXES
PROTEIN STRUCTURE
PROTEINS
REACTIVITY
SCATTERING
SPECIFICITY
SPECTRA
STABLE ISOTOPES
STRUCTURAL MODELS
SULFUR COMPOUNDS
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
TUNA
VERTEBRATES
X-RAY DIFFRACTION