Protein content and enzyme activities in methanol- and acetate-grown Methanosarcina thermophila

Journal Article · Wed Feb 28 23:00:00 EST 1990 · Journal of Bacteriology; (USA)

OSTI ID:6941327

Jablonski, P E; Cabell, M C; Ferry, J G ^[1]; DiMarco, A A; Bobik, T A ^[2]

---

1. Virginia Polytechnic Institute and State Univ., Blacksburg (USA)
2. Univ. of Illinois Urbana-Champaign (USA)

The cell extract protein content of acetate-and methanol-grown Methanosarcina thermophila TM-1 was examined by two-dimensional polyacrylamide gel electrophoresis. More than 100 mutually exclusive spots were present in acetate- and methanol-grown cells. Spots corresponding to acetate kinase, phosphotransacetylase, and the five subunits of the carbon monoxide dehydrogenase complex were identified in acetate-grown cells. Activities for formylmethanofuran dehydrogenase, formylmethanofurn:tetrahydromethanopterin formyl-transferase, 5,10-methenyltetrahydromethanopterin cyclohydrolase, methylene tetrahydromethanopterin:co-enzyme F{sub 420} oxidoreductase, formate dehydrogenase, and carbonic anhydrase were examined in acetate- and methanol-grown Methanosarcina thermophila. Levels of formyltransferase in either acetate- or methanol-grown Methanosarcina thermophila were approximately half the levels detected in H{sub 2}-CO{sub 2}-grown Methanobacterium thermoautotrophicum. All other enzyme activities were significantly lower in acetate- and methanol-grown Methanosarcina thermophila.

• DOE Contract Number: FG05-87ER13730
• OSTI ID: 6941327
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:3; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALCOHOLS
BACTERIA
BIOSYNTHESIS
CARBON-OXYGEN LYASES
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
HYDRO-LYASES
HYDROXY COMPOUNDS
LYASES
METABOLISM
METHANOGENIC BACTERIA
METHANOL
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PROTEINS
REDOX REACTIONS
SYNTHESIS
TRANSFERASES