Site-specific aflatoxin B sub 1 adduction of sequence-positioned nucleosome core particles
Thesis/Dissertation
·
OSTI ID:6940418
The question of how the presence of nucleosomal packing of DNA modifies carcinogen interaction at specific sites cannot be answered by studies on whole chromatin or bulk nucleosomes because of the heterogeneity of DNA sequences in the particles. This problem was circumvented by constructing nucleosomes that are homogenous in DNA-histone contact points. A cloned DNA fragment, containing a sea urchin 5S gene which precisely positions a histone octamer was employed. By using {sup 32}P end-labeled DNA and genotoxins that allow cleavage at sites of attack, the frequency of adduction at every susceptible nucleotide can be determined on sequencing gels. The small methylating agent dimethyl sulfate (DMS) and the bulky alkylating agent afatoxin B{sub 1}-dichloride (AFB{sub 1}-Cl{sub 2}) were used to probe the influence of DNA-histone interactions on DNA alkylation patterns in sequence-positioned core particles.
- Research Organization:
- Oregon State Univ., Corvallis, OR (USA)
- OSTI ID:
- 6940418
- Country of Publication:
- United States
- Language:
- English
Similar Records
Linker histones H1 and H5 prevent the mobility of positioned nucleosomes
Ultraviolet damage and nucleosome folding of the 5S ribosomal RNA gene.
Selective visualization of gene structure with ultraviolet light
Journal Article
·
Tue Oct 25 00:00:00 EDT 1994
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:6701355
Ultraviolet damage and nucleosome folding of the 5S ribosomal RNA gene.
Journal Article
·
Mon Jan 24 23:00:00 EST 2000
· Biochemistry
·
OSTI ID:15003117
Selective visualization of gene structure with ultraviolet light
Journal Article
·
Sun Jan 31 23:00:00 EST 1988
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5314298
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
ALKYLATION
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARCINOGENS
CHEMICAL REACTIONS
CHROMATIN
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ECHINODERMS
GENES
HISTONES
HYBRIDIZATION
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
NUCLEI
NUCLEOPROTEINS
NUCLEOSOMES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SEA URCHINS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
ALKYLATION
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARCINOGENS
CHEMICAL REACTIONS
CHROMATIN
CLONING
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ECHINODERMS
GENES
HISTONES
HYBRIDIZATION
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
NUCLEI
NUCLEOPROTEINS
NUCLEOSOMES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SEA URCHINS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES