Changes in the sup 14 C-labeled cell wall components with chase time after incorporation of UDP( sup 14 C)glucose by intact cotton fibers

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.86.4.1270· OSTI ID:6937728

Dugger, W M; Palmer, R L ^[1]

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1. Univ. of California, Riverside (USA)

Intact, in vitro-grown cotton fibers will incorporate ({sup 14}C)glucose from externally supplied UDP({sup 14}C)glucose into a variety of cell wall components including cellulose; this labeled fraction will continue to increase up to 4 hours chase time. In the fraction soluble in hot water there was no significant change in total label; however, the largest fraction after the 30 minute pulse with UDP({sup 14}C)glucose was chloroform-methanol soluble (70%) and showed a significant decrease with chase. The lipids that make up about 85% of this fraction were identified by TLC as steryl glucosides, acylated steryl glucosides, and glucosyl-phosphoryl-polyprenol. Following the pulse, the loss of label from acylated steryl glucosides and glucosylphophoryl-polyprenol was almost complete within 2 hours of chase; steryl glucosides made up about 85% of the fraction at that chase time. The total loss in the lipid fraction (about 100 picomoles per milligram dry weight of fiber) with chase times of 4 hours approximates the total gain in the total glucans.

• OSTI ID: 6937728
• Journal Information: Plant Physiology; (USA), Vol. 86:4; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL WALL
PHYSIOLOGY
GLUCOSE
BIOSYNTHESIS
LIPIDS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MARKERS
CARBON 14 COMPOUNDS
COTTON
FIBERS
GLYCOSIDES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
UDPG
ALDEHYDES
CARBOHYDRATES
CELL CONSTITUENTS
CHROMATOGRAPHY
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
551001* - Physiological Systems- Tracer Techniques