Solubilization of human platelet vasopressin receptors
Journal Article
·
· Life Sci.; (United States)
The human platelet membrane receptor for vasopressin (AVP) has been solubilized with the cholic acid derivative detergent 3-((3-cholamidopropyl)-dimethylammonio)-1-propane sulfonate. Rapid and simple separation of free tritiated AVP ((/sup 3/H)AVP) from the solubilized receptor-hormone complex was done by filtration through polyethylenimine-treated filters. (/sup 3/H)AVP binds to this soluble receptor with an equilibrium dissociation constant of 11.03 +/- 1.86 nM and a maximal number of binding sites = 288 +/- 66 fmol/mg protein while the corresponding values of the membrane-bound receptor are 1.62 +/- 0.21 nM and 237 +/- 38 fmol/mg of protein, respectively. The Ki value for native AVP derived from competition experiments is 11.02 +/- 20.5 nM for the soluble receptor. Competition experiments with specific vascular and renal antagonists confirm that the solubilized receptor belongs to the V1-vascular subtype. 10 references, 5 figures.
- Research Organization:
- Medical College of Ohio, Toledo
- OSTI ID:
- 6937721
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 40:5; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
DATA
EXPERIMENTAL DATA
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
REACTION KINETICS
RECEPTORS
SOLUBILITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOPRESSIN
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
DATA
EXPERIMENTAL DATA
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
REACTION KINETICS
RECEPTORS
SOLUBILITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOPRESSIN
VERTEBRATES