Measurement of human lung absorption of metaproterenol given via an aerosol using a stable isotope approach

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6937469

MacGregor, T R

The extent and rate of lung absorption of metaproterenol given via a metered dose aerosol was studied in normal, asthmatic, and chronic bronchitic volunteers. Aerosols of metaproterenol-d/sub 0/ and metaproterenol-/sup 13/C/sub 3/, 650 mcg doses each, were utilized followed in 3 hours by a 980 mcg intravenous infusion of metaproterenol-d/sub 7/. Pharmacokinetics of all three stable isotope formulations were studied in a 24 hour period in conjunction with pharmacologic effect measurements. A gas chromatographic/mass spectrometric assay for metaproterenol capable of reliably measuring plasma concentrations as low as 50 pg/mL was developed. Extent of lung absorption of metaproterenol following aerosol administration averaged 3% in normal and asthmatic volunteers (N = 6 each) and 1.8% in chronic bronchitic volunteers (N = 5). The extent absorbed on the second dose was comparable to the extent from the first dose. The rates of lung absorption, calculated by the Loo-Riegelman method, were bi-phasic in all volunteers with an initial, rapid first order phase followed by a slower, zero order phase. A working hypothesis, based on the solid suspension properties of the aerosol, was presented.

• Research Organization: Connecticut Univ., Storrs (USA)
• OSTI ID: 6937469
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SYMPATHOMIMETICS
UPTAKE
AEROSOLS
ASTHMA
BLOOD PLASMA
BRONCHITIS
CARBON 13
CHEMOTHERAPY
GAS CHROMATOGRAPHY
IN VIVO
LABELLED COMPOUNDS
LUNGS
MASS SPECTROSCOPY
TRACER TECHNIQUES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBON ISOTOPES
CHROMATOGRAPHY
COLLOIDS
DISEASES
DISPERSIONS
DRUGS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MATERIALS
NUCLEI
ORGANS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SEPARATION PROCESSES
SOLS
SPECTROSCOPY
STABLE ISOTOPES
THERAPY
551000* - Physiological Systems