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Lactate oxidation by three segments of the rabbit proximal tubule

Journal Article · · Proc. Soc. Exp. Biol. Med.; (United States)
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Oxidation of (U/sup 14/C)lactate to /sup 14/CO/sub 2/ was measured in vitro, in nonperfused anatomically defined segments of rabbit proximal tubule (S1, proximal convoluted, and S2 and S3, proximal straight tubules). The rate of lactate oxidation was similar in S2 and S3 segments, and within the range of lactate oxidation rates measured in vivo. In contrast, the oxidation rate of S1 segments was significantly lower than that of S2 or S3. In proximal straight tubules, lactate oxidation was inhibited by incubation at 0/sup 0/C, or by application of 1 mM ouabain. To determine if the rate of transepithelial transport affected the rate of lactate oxidation, lactate oxidation was measured in proximal straight tubules after the lumen had been opened by perfusion with Ringer's containing 10 mM polyethylene glycol. No difference in lactate oxidation rate was observed between tubules with patent lumina and nonperfused tubules. These results suggest that the various segments of the renal proximal tubule have difference metabolic characteristics, and that the rate of substrate oxidation is related to the activity of the Na/sup +/, K/sup +/-ATPase.
Research Organization:
Medical College of Ohio, Toledo
OSTI ID:
6937311
Journal Information:
Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 182:4; ISSN PSEBA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ANIMALS
ATP-ASE
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHALCOGENIDES
CHEMICAL REACTIONS
DRUGS
ENZYME ACTIVITY
ENZYMES
GLYCOSIDES
HYDROLASES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
LACTATES
MAMMALS
METABOLISM
ORGANIC COMPOUNDS
ORGANS
OUABAIN
OXIDATION
OXIDES
OXYGEN COMPOUNDS
PHOSPHOHYDROLASES
RABBITS
REACTION KINETICS
STROPHANTHINS
VERTEBRATES