Chinese hamster pleiotropic multidrug-resistant cells are not radioresistant

Journal Article · Fri Jan 01 04:00:00 EST 1988 · NCI Monogr.; (United States)

OSTI ID:6937020

Mitchell, J B; Gamson, J; Russo, A; Friedman, N; DeGraff, W; Carmichael, J; Glatstein, E

The inherent cellular radiosensitivity of a Chinese hamster ovary pleiotropic cell line that is multidrug resistant (CHRC5) was compared to that of its parental cell line (AuxB1). Radiation survival curve parameters n and D0 were 4.5 and 1.1 Gy, respectively, for the CHRC5 line and 5.0 and 1.2 Gy, respectively, for the parental line. Thus, the inherent radiosensitivity of the two lines was similar even though key intracellular free radical scavenging and detoxifying systems employing glutathione, glutathione transferase, and catalase produced enzyme levels that were 2.0-, 1.9-, and 1.9-fold higher, respectively, in the drug-resistant cell line. Glutathione depletion by buthionine sulfoximine resulted in the same extent of aerobic radiosensitization in both lines (approximately 10%). Incorporation of iododeoxyuridine into cellular DNA sensitized both cell lines to radiation. These studies indicate that pleiotropic drug resistance does not necessarily confer radiation resistance.

• Research Organization: National Cancer Institute, Bethesda, MD (USA)
• OSTI ID: 6937020
• Journal Information: NCI Monogr.; (United States), Journal Name: NCI Monogr.; (United States) Vol. 6; ISSN NCIME
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CHO CELLS
DNA
DRUGS
GLUTATHIONE
HAMSTERS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADICALS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RODENTS
SURVIVAL CURVES
VERTEBRATES