Comparative effects of endothelin and phorbol 12-13 dibutyrate in rat aorta

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90580-8· OSTI ID:6936423

Auguet, M; Delaflotte, S; Chabrier, P E; Braquet, P ^[1]

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1. Institut Henri Beaufour Research Labs., Cedex (France)

The vasoconstrictive properties of endothelin (ET-1) and the protein kinase C activator, phorbol 12-13 dibutyrate (PDB) were comparatively investigated in isolated rat aorta. ET-1 and PDB induced a slowly developing sustained contraction in endothelium denuded aorta. Maximal contractions induced by ET-1 and PDB were unaffected by diltiazem. Substantial contraction to ET-1 and PDB remained in calcium-free medium. Contractions of ET-1 and PDB in calcium-free medium were unaffected by intracellular calcium depletion induced by phenylephrine. Following the response to ET-1 and PDB in a calcium-free medium, an additional sustained was observed after calcium was added to the bath. The protein kinase C inhibitor, H7 was more potent in inhibiting contractions induced by phenylephrine and KCl than the ones elicited by ET-1 and PDB. The other protein kinase C inhibitors i.e. staurosporine and phloretin inhibited to a similar extent all the agonists tested. These results suggest that protein kinase C may play an important role in mediating the contraction to ET-1 in rat aorta.

• OSTI ID: 6936423
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 45:21; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
AORTA
ARTERIES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BLOOD VESSELS
BODY
CALCIUM
CARCINOGENS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
COMPARATIVE EVALUATIONS
DRUGS
ELEMENTS
ENDOTHELIUM
ENZYME INHIBITORS
ENZYMES
ESTERS
FUNCTIONS
INHIBITION
MAMMALS
METALS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RATS
RODENTS
TISSUES
TRANSFERASES
VASOCONSTRICTION
VASOCONSTRICTORS
VERTEBRATES