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Phosphorylation of the insulin receptor in cultured hepatoma cells and a solubilized system

Journal Article · · Methods Enzymol.; (United States)
OSTI ID:6936405
; ;

Methods are described which have been used successfully to study insulin receptor autophosphorylation in cultured cells (hepatoma cell line Fao) and detergent solubilized receptor systems. Intact cultured cells were labelled with /sup 32/PO/sub 4//sup 3 -/. Details are given for the solubilization and purification of the insulin receptor and insulin dose-response curves for phosphorylation of the solubilized insulin receptor. Trypsin digestion of a phosphorylated subunit suggests that at least peptides containing sites of /sup 32/P incorporation exist in the receptor molecule.

OSTI ID:
6936405
Journal Information:
Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
EXPERIMENTAL DATA
HEPATOMAS
HORMONES
HYDROLASES
INFORMATION
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIVER CELLS
MEMBRANE PROTEINS
NEOPLASMS
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDE HYDROLASES
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
POLYPEPTIDES
PROTEINS
PURIFICATION
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SERINE PROTEINASES
SOMATIC CELLS
TRACER TECHNIQUES
TRYPSIN