Alteration of extracellular enzymes in pinto bean leaves upon exposure to air pollutants, ozone and sulfur dioxide

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.1.159· OSTI ID:6936388

Peters, J L; Castillo, F J; Heath, R L ^[1]

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1. Univ. of California, Riverside (USA)

Diamine oxidase and peroxidase, associated with the wall in pinto bean (Phaseolus vulgaris L. var Pinto) leaves, can be washed out by vacuum infiltration and assayed without grinding the leaf. The diamine oxidase activity is inhibited in vivo by exposure of the plants to ozone (dose of 0.6 microliters per liter {times} hour), whereas the peroxidase activity associated with the wall space is stimulated. This dose does not cause obvious necrosis or chlorosis of the leaf. These alterations are greater when the dose of ozone exposure is given as a triangular pulse (a slow rise to a peak of 0.24 microliters per liter followed by a slow fall) compared to that given as a constant square wave pulse of 0.15 microliters per liter for the same 4 hour period. Exposure of the plants to sulfur dioxide (at a concentration of 0.4 microliters per liter for 4 hours) does not result in any change in the diamine oxidase or peroxidase activities, yet the total sulfhydryl content of the leaf is increased, demonstrating the entry of sulfur dioxide. These two pollutants, with different chemical reactivities, affect the activities of the extracellular enzymes in different manners. In the case of ozone exposure, the inhibition of extracellular diamine oxidase could profoundly alter the movements of polyamines from cell to cell.

• OSTI ID: 6936388
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 89:1; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
CHALCOGENIDES
ENZYME ACTIVITY
ENZYMES
INHIBITION
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OZONE
PEROXIDASES
PHASEOLUS
PLANTS
POLLUTION
SULFUR COMPOUNDS
SULFUR DIOXIDE
SULFUR OXIDES
TOXICITY