ADP-ribosylation of membrane components by pertussis and cholera toxin

Journal Article · Tue Jan 01 00:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09115-7· OSTI ID:6936308

Ribeiro-Neto, F A.P.; Mattera, F; Hildebrandt, J D; Codina, J; Field, J B; Birnbaumer, L; Sekura, R D

Pertussis and cholera toxins are important tools to investigate functional and structural aspects of the stimulatory (N/sub s/) and inhibitory (N/sub i/) regulatory components of adenylyl cyclase. Cholera toxin acts on N/sub s/ by ADP-ribosylating its ..cap alpha../sub s/ subunit; pertussis toxin acts on N/sub i/ by ADP-ribosylating its ..cap alpha..; subunit. By using (/sup 32/P)NAD/sup +/ and determining the transfer of its (/sup 32/P)ADP-ribose moiety to membrane components, it is possible to obtain information on N/sub s/ and N/sub i/. A set of protocols is presented that can be used to study simultaneously and comparatively the susceptibility of N/sub s/ and N/sub i/ to be ADP-ribosylated by cholera and pertussis toxin.

• OSTI ID: 6936308
• Journal Information: Methods Enzymol.; (United States), Vol. 109
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYCLASES
ENZYME ACTIVITY
TOXINS
BIOCHEMICAL REACTION KINETICS
ADP
CELL MEMBRANES
CHOLERA
EXPERIMENTAL DATA
LABELLED COMPOUNDS
NAD
PHOSPHORUS 32
RIBOSE
TRACER TECHNIQUES
ALDEHYDES
ANTIGENS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
COENZYMES
DATA
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
INFECTIOUS DISEASES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LYASES
MATERIALS
MEMBRANES
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PENTOSES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques