Kinetics of p-nitrophenol mineralization by a Pseudomonas sp. : effects of sound substrates
The kinetics of simultaneous mineralization of p-nitrophenol (PNP) and glucose by Pseudomonas sp. were evaluated by nonlinear regression analysis. Pseudomonas sp. did not mineralize PNP at a concentration of 10 ng/ml but metabolized it at concentrations of 50 ng/ml or higher. The K/sub s/ value for PNP mineralization by Pseudomonas sp. was 1.1 ..mu..g/ml, whereas the K/sub s/ values for phenol and glucose mineralization were 0.10 and 0.25 ..mu..g/ml respectively. The addition of glucose to the media did not enable Pseudomonas sp. to mineralize 10 ng of PNP per ml but did enhance the degradation of higher concentrations of PNP. This enhanced degradation resulted from the simultaneous use of glucose and PNP and the increased rate of growth of Pseudomonas sp. on glucose. The Monod equation and a dual-substrate model fit these data equally well. The dual-substrate model was used to analyze the data because the theoretical assumptions of the Monod equation were not met. Phenol inhibited PNP mineralization and changed the kinetics of PNP mineralization so that the pattern appeared to reflect growth, when in fact growth was not occurring. Thus, the fitting of models to substrate depletion curves may lead to erroneous interpretations of data if the effects of second substrates on population dynamics are not considered.
- Research Organization:
- Cornell Univ., Ithaca, NY (USA)
- OSTI ID:
- 6936102
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:11; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
AROMATICS
BACTERIA
BIODEGRADATION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
EQUATIONS
GLUCOSE
GROWTH
HEXOSES
HYDROXY COMPOUNDS
MATHEMATICAL MODELS
MATHEMATICS
METABOLISM
MICROORGANISMS
MONOSACCHARIDES
NITRO COMPOUNDS
NITROPHENOL
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
PSEUDOMONAS
REGRESSION ANALYSIS
SACCHARIDES
STATISTICS
SUBSTRATES