Cadmium accumulation by immobilized cells of a Citrobacter sp. using various phosphate donors

Journal Article · Mon Sep 01 04:00:00 EDT 1986 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260280910· OSTI ID:6936070

Michel, L J; Macaskie, L E; Dean, A C.R.

Immobilized cells of a Citrobacter species scavenge cadmium with high efficiency from challenge flows containing CD/sup 2 +/. Metal uptake by the cells is mediated by a cell-bound phosphatase which liberates inorganic phosphate from an organic phosphate to precipitate cadmium as cell-bound metal phosphate. Hitherto glycerol 2-phosphate has served as the phosphate donor, but for an economic large scale process an inexpensive and readily available phosphate donor is required and the use of alkyl phosphates was investigated. This was limited due to interference by the alcohol simultaneously liberated. An alternative, pulsed process is described whereby alkyl phosphate-supplemented main pulses interspersed with short alkyl phosphate-free recovery pulses greatly reduced the requirement for glycerol 2-phosphate. Wider aspects of phosphate donor utilization were also investigated to compare this strain of Citrobacter with a strain previously reported to accumulate lead but not cadmium.

• Research Organization: Univ. of Oxford, England
• OSTI ID: 6936070
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:9; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

550500* -- Metabolism
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL ACCUMULATION
CADMIUM
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ESTERASES
HYDROLASES
IMMOBILIZED CELLS
METABOLISM
METALS
MICROORGANISMS
NONMETALS
PERFORMANCE
PHOSPHATASES
PHOSPHORUS
RESPONSE MODIFYING FACTORS
SCAVENGING
UPTAKE