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Sister chromatid exchange analysis in lung and peripheral blood lymphocytes of mice exposed to methyl isocyanate by inhalation

Journal Article · · Environ. Mutagen.; (United States)
Mice were exposed to 1, 3, or 6 ppm methyl isocyanate (MIC) for 6 hr/day for four consecutive days. Lung cells and peripheral blood lymphocytes (PBLs) were removed and cultured for analysis of sister chromatid exchange (SCE) and cell cycle kinetics. MIC caused a small but significant increase in SCE frequency of cultured lung cells from mice exposed to 1, 3, or 6 ppm MIC. MIC did not significantly increase SCE levels in PBLs of mice exposed to concentrations as high as 6 ppm. In cultured PBLs, MIC had a stimulatory effect on cell cycling rates as measured by the replicative index, and it caused a significant reduction in mononuclear leucocyte counts and the mitotic indices.
Research Organization:
Environmental Health Research and Testing, Inc., Research Triangle Park, NC
OSTI ID:
6934723
Journal Information:
Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 9:1; ISSN ENMUD
Country of Publication:
United States
Language:
English