Labeling of pseudomonas aeruginosa with In-111-oxine
Labeling of live bacteria with gamma emitting radioisotope provides a useful tool for the experimental in vivo tracking of bacteria in various body organs of animals. The authors labeled a serum resistant strain of Pseudomonas aeruginosa (ATCC number27853) with In-111-oxine. P. aeruginosa streaked heavily on ten blood agar plates, was grown overnight, and suspended in 50 ml of saline using sterile cotton swabs. The suspension was sonicated for 3 minutes at 40 watts with a small probe, 500 ..mu..Ci of commercially prepared In-111-oxine added and the bacteria incubated at 37/sup 0/C for 2.5 hours. The labeled bacteria were centrifuged and washed once with saline and resuspended to a final volume of 50 ml in saline. The labeled Pseudomonas, 10/sup 9/-10/sup 10/ cfu/ml, retained 120-190 ..mu..Ci of cell-bound In-111. In vitro studies showed good retention of the In-111 label in saline at 37/sup 0/C (75-85% cell-bound radioactivity at 1 hour) and in canine blood at 37/sup 0/C (30-55% cell-bound radioactivity at 1 hour). The loss of cell-associated radioactivity in blood, with a corresponding decrease in the number of viable organisms, is probably a result of phagocyte-mediated killing of the organisms and subsequent release of the label. The labeled bacteria have been used successfully for sequential imaging in experimental animals to track bacteria injected into blood and the biliary tree.
- Research Organization:
- Minneapolis VA Medical Center, Minneapolis, MN
- OSTI ID:
- 6933887
- Report Number(s):
- CONF-840619-; TRN: 87-008766
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 25:5; Conference: 31. annual meeting of the Society of Nuclear Medicine, Los Angeles, CA, USA, 5 Jun 1984
- Country of Publication:
- United States
- Language:
- English
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BILIARY TRACT
RADIOISOTOPE SCANNING
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DIAGNOSTIC USES
OXINE
PSEUDOMONAS
LABELLING
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GAMMA RADIATION
IN VIVO
INJECTION
MEDIUM TEMPERATURE
TRACER TECHNIQUES
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MAMMALS
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550601* - Medicine- Unsealed Radionuclides in Diagnostics