Cadmium-binding proteins from blue crabs (Callinectes sapidus) environmentally exposed to cadmium
Two heat-stable (90/sup 0/C) cadmium-binding proteins were isolated from the hepatopancreas of Hudson River blue crabs (Callinectes sapidus) by Sephadex G-75 gel filtration chromatography. These proteins have molecular weights of 10,600 and 9,400, and ultraviolet absorbance ratios at 250/280 nm of 12.4 and 5.4, respectively. Repeated freezing and thawing and prolonged (3-6 weeks) storage resulted in protein degradation or loss of Cd-binding activity. These proteins were induced by laboratory injection of CdCl/sub 2/ in blue crabs from pristine (Chesapeake Bay) areas; however, injection of CdCl/sub 2/ into Hudson River animals yielded anomalous chromatography profiles. Cadmium-binding proteins were also identified in blue crab thoracic muscle and gill. The possibility is discussed that these proteins are a type of metallothionein and could contribute to the human toxicity of this cadmium-contaminated edible crustacean.
- Research Organization:
- New York Univ. Medical Center, New York
- OSTI ID:
- 6933787
- Journal Information:
- Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 28:1; ISSN ENVRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
BIOLOGICAL ACCUMULATION
BODY
CADMIUM
CHEMICAL BONDS
CHROMATOGRAPHY
CONTAMINATION
CRUSTACEANS
DIGESTIVE SYSTEM
ELEMENTS
ENDOCRINE GLANDS
GILLS
GLANDS
INVERTEBRATES
LIVER
METALLOPROTEINS
METALLOTHIONEIN
METALS
MUSCLES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PROTEINS
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
SEPARATION PROCESSES
TOXICITY