Radioimmunoassay of a thymine glycol
Journal Article
·
· Radiat. Res.; (United States)
A sensitive and specific radioimmunoassay for 5,6-dihydroxy-5,6-dihydrothymine (thymine glycol) was developed. As lttle as 4 femtomoles of thymine glycol can be detected in OsO/sub 4/-treated DNA. Unmodified DNA cross-reacts only at the highest concentrations. The assay is dependent on both the absolute amount of thymine glycol and the fraction of thymine altered in the polymer. This is caused by the high specificity of the antibody for multiple thymine glycol sites, as confirmed using OsO/sub 4/-modified poly(dA-dT) and a series of oligothymidylic acids. A method of correcting for this dependence is presented. Using this RIA thymine glycol is detected in ..gamma..-irradiated DNA at a G value of 0.018; the reasons for this low yield are discussed. The assay requires only mild chemical treatment and many samples can be quickly and easily handled.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 6932786
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALCOHOLS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHALCOGENIDES
COBALT 60
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OSMIUM COMPOUNDS
OSMIUM OXIDES
OXIDES
OXYGEN COMPOUNDS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYMINE
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
URACILS
YEARS LIVING RADIOISOTOPES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALCOHOLS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHALCOGENIDES
COBALT 60
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OSMIUM COMPOUNDS
OSMIUM OXIDES
OXIDES
OXYGEN COMPOUNDS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
THYMINE
TRACER TECHNIQUES
TRANSITION ELEMENT COMPOUNDS
URACILS
YEARS LIVING RADIOISOTOPES