Photoreactivation and excision repair of thymine dimers in ultraviolet-irradiated cultured fish cells
Journal Article
·
· Radiat. Res.; (United States)
It was confirmed by colony-formation assays that cultured fish cells (CAF-MM1) are more sensitive to ultraviolet light (uv) than mammalian cells (mouse L cells). To study the high sensitivity of the fish cells, direct assay of thymine dimers was made by two-dimensional paper chromatography. The ratio of thymine dimers to thymine (TT/T) immediately after uv irradiation was proportional to uv dose and was 0.04% per 10 J/m/sup 2/ of uv. Similar values were also obtained in the mouse L cells used for comparison. When CAF-MM1 cells were incubated in the dark after uv irradiation, the value of TT/T did not change for up to 48 hr; that is, excision of dimers could not be detected. Visible light illumination immediately after uv irradiation reduced the amount of thymine dimers and increased the cell survival of CAF-MM1 cells. The results indicated a close relation between photoreactivation of dimers and the fraction of cells surviving. About half the thymine dimers could be photoreactivated upon exposure to visible light immediately after uv irradiation, but only 18% after 24 hr.
- Research Organization:
- Univ. of Tokyo, Japan
- OSTI ID:
- 6929214
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Survival and photoreactivability of ultraviolet-irradiated cultured fish cells (CAF-MM1)
Effects of microinjected photoreactivating enzyme on thymine dimer removal and DNA repair synthesis in normal human and xeroderma pigmentosum fibroblasts
Problems concerning thymine dimer assay by the radiochromatographic method
Journal Article
·
Sun Nov 30 23:00:00 EST 1980
· Radiat. Res.; (United States)
·
OSTI ID:6688766
Effects of microinjected photoreactivating enzyme on thymine dimer removal and DNA repair synthesis in normal human and xeroderma pigmentosum fibroblasts
Journal Article
·
Wed Mar 14 23:00:00 EST 1990
· Cancer Research; (USA)
·
OSTI ID:6853782
Problems concerning thymine dimer assay by the radiochromatographic method
Journal Article
·
Sun Dec 31 23:00:00 EST 1972
· Stud. Biophys., v. 36-37, pp. 43-50
·
OSTI ID:4393324
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DIMERS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FISHES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOREACTIVATION
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SURVIVAL CURVES
THYMINE
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
VISIBLE RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DIMERS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
FISHES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOREACTIVATION
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SURVIVAL CURVES
THYMINE
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
VISIBLE RADIATION