Induction of L-arabinose isomerase in gamma-irradiated Escherichia coli

Journal Article · Sat Nov 01 00:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576824· OSTI ID:6928093

Chatterjee, A; Bhattacharya, A K

Gamma irradiation of Escherichia coli B/r caused a dose-dependent inhibition of the capacity of the cells to synthesize L-arabinose isomerase in response to the inducer. At higher doses (18 krad and above), postirradiation incubation led to further inhibition of the capacity to synthesize L-arabinose isomerase, whereas cells receiving lower doses recovered from the damage to the enzyme synthesizing system following incubation. Cyclic AMP partially reversed the inhibitory effect on L-arabinose isomerase induction produced immediately after irradiation by all gamma-ray doses (up to 30 krad), but the enhanced inhibitory effect caused by induction in cells irradiated at higher doses could not be reversed by the nucleotide. It is suggested that although catabolite repression is partly responsible for causing the inhibition of the enzyme synthesizing capacity of the cells observed immediately after gamma irradiation, the enhanced inhibition caused by incubating cells irradiated at higher doses is not due to interference with the control mechanism regulated by catabolite repression.

• Research Organization: Banaras Hindu Univ., Varanasi, India
• OSTI ID: 6928093
• Journal Information: Radiat. Res.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ESCHERICHIA COLI
BIOLOGICAL RADIATION EFFECTS
ISOMERASES
ENZYME INDUCTION
ARABINOSE
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
ALDEHYDES
BACTERIA
BIOLOGICAL EFFECTS
CARBOHYDRATES
ELECTROMAGNETIC RADIATION
ENZYMES
GENE REGULATION
IONIZING RADIATIONS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
560130* - Radiation Effects on Microorganisms