Manipulatiaon of Biofilm Microbial Ecology
- ORNL
The Biofilm mode of growth provides such significant advantages to the members of the consortium that most organisms in important habitats are found in biofilms. The study of factors that allow manipulation of biofilm microbes in the biofilm growth state requires that reproducible biofilms by generated. The most effective monitoring of biofilm formation, succession and desquamation is with on-line monitoring of microbial biofilms with flowcell for direct observation. The biofilm growth state incorporates a second important factor, the heterogeneity in the distribution in time and space of the component members of the biofilm consortium. This heterogeneity is reflected not only in the cellular distribution but in the metabolic activity within a population of cells. Activity and cellular distribution can be mapped in four dimensions with confocal microscopy, and function can be ascertained by genetically manipulated reporter functions for specific genes or by vital stains. The methodology for understanding the microbial ecology of biofilms is now much more readily available and the capacity to manipulate biofilms is becoming an important feature of biotechnology.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
- Sponsoring Organization:
- National Library of medicine; National Water Research Institute; Natural and Accelerated Bioremediation Program; USDOE Office of Science
- DOE Contract Number:
- AC05-96OR22464
- OSTI ID:
- 6927
- Report Number(s):
- ORNL/CP-102998; 26 42 00 00 0; ON: DE00006927
- Country of Publication:
- United States
- Language:
- English
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