Benzidine: mechanisms of oxidative activation and mutagenesis

Conference · Mon Sep 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6926981

Josephy, P D

Benzidine oxidative activation may proceed by peroxidase-catalyzed one-electron oxidation via free radical intermediates, or by N-acetylation followed by monooxygenase-catalyzed N-hydroxylation. The peroxidase route has been examined by using horseradish peroxidase or prostaglandin H synthase in vitro. In the presence of nucleophiles such as phenols, thiols, or nucleic acids, isolable adducts are formed. The structures of these adducts have been elucidated by spectroscopic methods. The Ames test provides a useful system for studying benzidine bioactivation to mutagenic intermediates. An endogenous bacterial actylase plays an important auxiliary role in the hepatic S9-dependent activation of benzidine. Bacterial peroxidases may also support benzidine oxidation in the Ames test.

• Research Organization: Univ. of Guelph, Ontario
• OSTI ID: 6926981
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:10
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AROMATICS
BACTERIA
BENZIDINE
ENZYMES
LIGASES
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
SALMONELLA
SCREENING
TOXICITY