Pertussis toxin treatment attenuates some effects of insulin in BC3H-1 murine myocytes
The effects of pertussis toxin (PT) treatment on insulin-stimulated myristoyl-diacylglycerol (DAG) generation, hexose transport, and thymidine incorporation were studied in differentiated BC3H-1 mycocytes. Insulin treatment caused a biphasic increase in myristoyl-DAG production which was abolished in myocytes treated with PT. There was no effect of PT treatment on basal (nonstimulated) myristoyl-DAG production. Insulin-stimulated hydrolysis of a membrane phosphatidylinositol glycan was blocked by PT treatment. ADP-ribosylation of BC3H-1 plasma membranes with (/sup 32/P)NAD revealed a 40-kDa protein as the major PT substrate in vivo and in vitro. The time course and dose dependence of the effects of PT on diacylglycerol generation correlated with the in vivo ADP-ribosylation of the 40-kDa substrate. Pertussis toxin treatment resulted in a 71% attenuation of insulin-stimulated hexose uptake without effect on either basal or phorbol ester-stimulated uptake. The stimulatory effects of insulin and fetal calf serum on (/sup 3/H)thymidine incorporation into quiescent myocytes were attenuated by 61 and 59%, respectively, when PT was added coincidently with the growth factors. Nonstimulated and EGF-stimulated (/sup 3/H)thymidine incorporation was unaffected by PT treatment. These data suggest that a PT-sensitive G protein is involved in the cellular signaling mechanisms of insulin.
- Research Organization:
- Univ. of Virginia School of Medicine, Charlottesville (USA)
- OSTI ID:
- 6925014
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:13; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMAL CELLS
ANIMALS
ANTIGENS
AZINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL DIFFERENTIATION
CHEMISTRY
DOSE-RESPONSE RELATIONSHIPS
GLUCOSE
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
INSULIN
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICE
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
MUSCLES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PYRIMIDINES
RIBOSIDES
RODENTS
SACCHARIDES
TETRADECANOIC ACID
THYMIDINE
TOXIC MATERIALS
TOXINS
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES