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Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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Screening of a lambdagt11 human melanocyte cDNA library with antibodies against hamster tyrosinase resulted in the isolation of 16 clones. The cDNA inserts from 13 of the 16 clones cross-hybridized with each other, indicating that they were form related mRNA species. One of the cDNA clones, Pmel34, detected one mRNA species with an approximate length of 2.4 kilobases that was expressed preferentially in normal and malignant melanocytes but not in other cell types. The amino acid sequence deduced from the nucleotide sequence showed that the putative human tyrosinase is composed of 548 amino acids with a molecular weight of 62,610. The deduced protein contains glycosylation sites and histidine-rich sites that could be used for copper binding. Southern blot analysis of DNA derived from newborn mice carrying lethal albino deletion mutations revealed that Pmel34 maps near or at the c-albino locus, the position of the structural gene for tyrosinase.

Research Organization:
Guthrie Research Institute, Sayre, PA (USA)
OSTI ID:
6924696
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:21; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ENZYMES
EVEN-ODD NUCLEI
GENETICS
HYBRIDIZATION
HYDROXY COMPOUNDS
HYDROXYLASES
ISOTOPES
LETHAL MUTATIONS
LIGHT NUCLEI
MAMMALS
MAN
MELANIN
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PIGMENTS
PRIMATES
RADIOISOTOPES
RECOMBINANT DNA
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
TYROSINASE
VERTEBRATES