Membrane alterations following toxic chemical insult. Research progress report No. 3 (Final), 15 July 1984-31 January 1988
A procaryotic cell system was developed that can be used to determine the toxic action of chemicals acting at the level of the eucaryotic or procaryotic cytoplasmic membrane. Cell wall-less microbes known as mycoplasmas were used. In this current study, two perfluorinated fatty acids (CB and C10) were found to inhibit the growth of the test mycoplasmas. Two apparent activities, cytotoxicity and cytolysis, were observed. At high concentrations (>10 mM), a detergent-like action was noted. At low concentrations (<10 mM), cell death was observed without detectable cell lysis. Altering the cell membrane (the presumed target of the toxic compounds) resulted in altered levels to toxicity. Similar results were obtained when human or murine B-cells were used as the target organism. The toxic action of the perfluorinated fatty acids apparently involves some interaction with the membrane of the cells being treated.
- Research Organization:
- State Univ. of New York, Binghamton (USA). Dept. of Biological Sciences
- OSTI ID:
- 6923713
- Report Number(s):
- AD-A-194391/9/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYLIC ACIDS
ORGANIC FLUORINE COMPOUNDS
CYTOLOGY
TOXICITY
TOXIC MATERIALS
BIOLOGICAL EFFECTS
CELL MEMBRANES
CYTOPLASM
DEATH
MYCOPLASMA
PROGRESS REPORT
BIOLOGY
CELL CONSTITUENTS
DOCUMENT TYPES
MATERIALS
MEMBRANES
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
550900* - Pathology
550300 - Cytology