Use of anti-idiotypic antibodies to establish that monoclonal antibody 7H11D6 binds to the. cap alpha. /sub 2/-macroglobulin receptor recognition site
Studies were performed to determine if monoclonal antibody 7H11D6 binds to the region of ..cap alpha../sub 2/-macroglobulin (..cap alpha../sub 2/M) that interacts with cell-surface receptors. Chemical modification studies revealed that the epitope region for 7H11D6 is sensitive to modification of the inhibitor with cis-dichlorodiammineplatinum (II). To determine if the epitope for 7H11D6 is comprised of residues involved in the ..cap alpha../sub 2/M receptor determinant, anti-idiotypic antibodies against 7H11D6 were prepared. A competitive enzyme-linked immunosorbent assay demonstrated that the anti-idiotypic IgG competed with ..cap alpha../sub 2/M-trypsin complexes for binding to 7H11D6. The anti-idiotypic IgG inhibited the binding of /sup 125/I-labeled ..cap alpha../sub 2/M-trypsin complexes to normal rat kidney fibroblasts, and a K/sub d/ of 194 pM for the binding of the anti-idiotype to these cells was derived from a fit of the data to a model involving a single class of sites. Binding of the anti-idiotypic IgG to the ..cap alpha../sub 2/M receptor provides unequivocal evidence that 7H11D6 is binding to residues within the receptor recognition site on ..cap alpha../sub 2/M and not merely to residues sufficiently close to that region to cause steric hindrance.
- Research Organization:
- American Red Cross Biomedical Research and Development, Rockville, MD (USA)
- OSTI ID:
- 6922276
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 263:14; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evidence that the platinum-reactive methionyl residue of the. cap alpha. /sub 2/-macroglobulin receptor recognition site is not in the carboxyl-terminal receptor binding domain
Anti-idiotypic antibody to an anti-alprenolol monoclonal antibody: promotion of ligand binding to the idiotype
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CONFIGURATION INTERACTION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME IMMUNOASSAY
FIBROBLASTS
GLOBULINS
IMMUNOASSAY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MEMBRANE PROTEINS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PLATINUM COMPOUNDS
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SOMATIC CELLS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES