Use of isothiocyanatobenzyl-DTPA derivatized monoclonal antimyosin fab for enhanced in vivo target localization
- Massachusetts General Hospital, Boston (USA)
Monoclonal antimyosin Fab (AM-Fab) was radiolabeled with 111In via a new bifunctional chelating agent, isothiocyanatobenzyl-DTPA (SCN-DTPA), and used to visualize acute reperfused experimental myocardial infarction. Antibody localization was compared to {sup 201}Tl (0.6 mCi) distribution in nine animals. Each animal was injected intravenously with 0.5 mCi of {sup 111}In-SCN-DTPA AM-Fab preparations (Prep 1 (n = 5) and 2 (n = 4)). The biodistribution was compared to that of {sup 111}In-labeled conventional bicyclic anhydride DTPA-AM-Fab (n = 5). {sup 111}In-SCN-DTPA AM-Fab Prep 1 (lowest specific activity) showed highest specific target localization (31.6 +/- 3.5, MEAN infarct(0-20% Tl-201) to normal ration +/- SE) and lowest hepatic sequestration (0.0108 +/- 0.002% ID/g). Prep 2 showed similar infarct localization (18.4 +/- 1.2) to control {sup 111}In-DTPA AM-Fab (16.9 +/- 1.1), but had higher hepatic activity (0.0326 +/- 0.014 and 0.0267 +/- 0.006 respectively). This difference in in vivo localization occurred despite the lack of changes in in vitro immunoreactivities of the AM-Fab preparations. The enhanced target localization with minimal hepatic activity may permit a more sensitive diagnostic application of {sup 111}In-labeled AM-Fab in future clinical studies.
- OSTI ID:
- 6920860
- Journal Information:
- Journal of Nuclear Medicine; (USA), Journal Name: Journal of Nuclear Medicine; (USA) Vol. 31:2; ISSN 0161-5505; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
CARDIOVASCULAR DISEASES
CHELATING AGENTS
COMPARATIVE EVALUATIONS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HEAVY NUCLEI
IMMUNOGLOBULINS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
MYOCARDIAL INFARCTION
MYOSIN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOIMMUNODETECTION
RADIOIMMUNOSCINTIGRAPHY
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
SECONDS LIVING RADIOISOTOPES
SPECIFICITY
THALLIUM 201
THALLIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES