Bioluminescent reporter bacteria detect contaminants in soil samples
Journal Article
·
· Applied Biochemistry and Biotechnology; (United States)
- Oak Ridge National Lab., TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
Reporter strains of bacteria were tested using soil samples from several sites near a leaking fuel oil storage facility. The reporter bacteria utilized the bioluminescent lux genes from Vibrio fischeri, which were transcriptionally fused to catabolic gene sequences. The catabolic genes of interest specified the degradation of toluene (from the TOL plasmid) and naphthalene (from the NAH7 plasmid and from a NAH plasmid recently isolated). The results indicated that two soil samples were contaminated with both toluene (or xylene) and naphthalene. These data were useful in describing the extent of contamination at the site. 9 refs., 5 figs., 1 tab.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 6917050
- Journal Information:
- Applied Biochemistry and Biotechnology; (United States), Journal Name: Applied Biochemistry and Biotechnology; (United States) Vol. 45-46; ISSN ABIBDL; ISSN 0273-2289
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulated Bioluminescence as a Tool for Bioremediation Process Monitoring and Control of Bacterial Cultures
Rapid, sensitive bioluminescent reporter technology for napthalene exposure and biodegradation
Molecular ecology of bacterial populations in environmental hazardous chemical control. Annual report, 15 January 1993-14 January 1994
Conference
·
Mon Dec 02 23:00:00 EST 1991
·
OSTI ID:10102868
Rapid, sensitive bioluminescent reporter technology for napthalene exposure and biodegradation
Journal Article
·
Fri Aug 17 00:00:00 EDT 1990
· Science (Washington, D.C.); (USA)
·
OSTI ID:6225865
Molecular ecology of bacterial populations in environmental hazardous chemical control. Annual report, 15 January 1993-14 January 1994
Technical Report
·
Thu Jan 13 23:00:00 EST 1994
·
OSTI ID:7286288
Related Subjects
54 ENVIRONMENTAL SCIENCES
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIOLUMINESCENCE
BIOTECHNOLOGY
CATABOLISM
CONDENSED AROMATICS
DETECTION
GENETIC ENGINEERING
HYDROCARBONS
LUMINESCENCE
METABOLISM
MICROORGANISMS
NAPHTHALENE
ORGANIC COMPOUNDS
POLLUTION
PSEUDOMONAS
SOILS
TOLUENE
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BIOLUMINESCENCE
BIOTECHNOLOGY
CATABOLISM
CONDENSED AROMATICS
DETECTION
GENETIC ENGINEERING
HYDROCARBONS
LUMINESCENCE
METABOLISM
MICROORGANISMS
NAPHTHALENE
ORGANIC COMPOUNDS
POLLUTION
PSEUDOMONAS
SOILS
TOLUENE