Elemental changes at irritant reactions due to chromate and nickel in guinea-pig epidermis

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Scanning Electron Microsc.; (United States)

OSTI ID:6914215

Lindberg, M; Forslind, B; Roomans, G M

The present investigation was performed to examine the possibility of using energy dispersive X-ray microanalysis (EDX) to obtain information on epidermal reactions at irritant dermatitis. The elemental redistribution in the stratum germinativum and stratum spinosum of the guinea-pig epidermis after intradermal injection of a chromate and a nickel solution was studied in thick cryo-sections. The two solutions caused different changes in elemental content in both cell layers. Nickel sulfate caused a conspicuous decrease in K levels whereas Na and Cl were elevated in both cell layers. Potassium chromate caused an increase in Cl but lowered the P values. The result of this study show that it is possible to use EDX-analysis of thick skin sections to obtain information on physiologically relevant alterations in epidermis at irritant dermatitis.

• Research Organization: Karolinska Inst., Stockholm, Sweden
• OSTI ID: 6914215
• Journal Information: Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States) Vol. 3; ISSN SEMYB
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AQUEOUS SOLUTIONS
BODY
CHEMICAL COMPOSITION
CHROMATES
CHROMIUM COMPOUNDS
DISPERSIONS
DISTRIBUTION
ELECTRON SPECTROSCOPY
ELEMENTS
GUINEA PIGS
INJECTION
INTAKE
MAMMALS
MEASURING METHODS
METALS
MIXTURES
NICKEL
ORGANS
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RODENTS
SKIN
SOLUTIONS
SPECTROSCOPY
SUBCUTANEOUS INJECTION
TISSUE DISTRIBUTION
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
VERTEBRATES