Monitoring exposure to acrylamide by the determination of S-(2-carboxyethyl)cysteine in hydrolyzed hemoglobin by gas chromatography-mass spectrometry

Journal Article · Mon Sep 01 00:00:00 EDT 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90621-4· OSTI ID:6914134

Bailey, E; Farmer, P B; Bird, I; Lamb, J H; Peal, J A

Acrylamide is a potent cumulative neurotoxin in animals and man. In vivo exposure to this electrophile results in the formation of a covalently bound reaction product with cysteine residues in hemoglobin. This adduct yields on acid hydrolysis S-(2-carboxyethyl)cysteine which has been analyzed by capillary gas chromatography with mass spectrometry. Globin isolated from the blood of rats exposed to acrylamide was spiked with an internal standard (globin treated in vitro with d3-acrylamide) and was then hydrolyzed with 6 N HCl. The protein hydrolysate was fractionated on a Dowex 50W H+ ion exchange column and the amino acids in the partially purified extract were determined as N-heptafluorobutyryl methyl esters using an OV-1701 fused silica capillary column. Quantitation was made by chemical ionization (isobutane) selective ion monitoring in which the ions m/z 386 (M-OCH3)+ derived from derivatized S-(2-carboxyethyl)cysteine in the sample and the corresponding ion m/z 389 from the added deuterium-labeled internal standard were monitored. The dose-response relationship between production of hemoglobin adduct and dose of acrylamide (0.1 mg/kg-5 mg/kg) is curved, showing an increasing slope with increasing doses of acrylamide.

• Research Organization: MRC Labs., Carshalton, England
• OSTI ID: 6914134
• Journal Information: Anal. Biochem.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRYLAMIDE
BIOLOGICAL INDICATORS
CYSTEINE
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
ADDUCTS
ENVIRONMENTAL EXPOSURE
HEMOGLOBIN
MONITORING
RATS
AMIDES
AMINO ACIDS
ANIMALS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RODENTS
SEPARATION PROCESSES
SPECTROSCOPY
THIOLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology