Biosensors based on polymer networks formed by gamma irradiation crosslinking
- Univ. of Cincinnati, OH (United States)
Water-soluble polymers immobilized by gamma radiation have been investigated as a means of developing electrochemical sensors. Enzyme-based sensors for glucose and lactate have been made by immobilizing glucose oxidase and lactate oxidase, respectively, on platinized graphite electrodes. The enzyme is entrapped in a polymeric network of poly(vinyl alcohol) that is formed by gamma radiation crosslinking. Electrodes coated with poly (N-vinylpyrrolidone) and its corresponding monomers and then crosslinked with gamma radiation show an extraction of catecholamines into the polymer film that enhances the analytical signal for their detection by electrochemical oxidation. Poly(dimethyldiallylammonium chloride) spin-coated on a screen-printed electrochemical cell provides sufficient ionic conductivity for the cell to function as a gas sensor for oxygen, which is detected by reduction at a platinum working electrode. 13 refs., 9 figs.
- OSTI ID:
- 6912802
- Journal Information:
- Applied Biochemistry and Biotechnology; (United States), Vol. 41:1-2; ISSN 0273-2289
- Country of Publication:
- United States
- Language:
- English
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ENZYMES
SURFACE COATING
PVA
PVP
CHEMICAL RADIATION EFFECTS
ALCOHOLS
AMIDES
AZOLES
BLOOD SUBSTITUTES
DEPOSITION
DRUGS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LACTAMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC POLYMERS
POLYMERS
POLYVINYLS
PROTEINS
PYRROLES
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RADIATION EFFECTS
440800* - Miscellaneous Instrumentation- (1990-)