Thylakoid phosphoproteins
Thylakoid phosphoproteins were successively fractionated by (1) treatment of /sup 32/P-labeled membranes with 1 M NaBr to remove superficial proteins; (2) extraction with octyl glucoside/cholate; (3) precipitation with ammonium sulfate; (4) size exclusion chromatography on BioGel P300, and (5) sucrose density gradient centrifugation. The detergent extract contained <10% of the original membrane-bound /sup 32/P; it was enriched in cytochrome b/f complex and 64-kDa protein kinase. A 20-kDa protein which copurified with the cytochrome complex and was assumed to be the Rieske protein, was partially phosphorylated. The protein kinase, which phosphorylates itself in vitro, appeared on the sucrose gradient as a phosphoprotein, signalling that it had become labeled in the intact thylakoid. A phosphoprotein of approx.10 kDa which is seen as a product of directly radiolabeling the BioGel P300 extract, was found to differ from the well documented phosphoprotein of this approximate mass that appears in labeled thylakoids. 30 refs., 3 figs., 2 tabs.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 6908178
- Report Number(s):
- BNL-38870; CONF-860808-11; ON: DE87003203
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
FRACTIONATION
GEL PERMEATION CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHOTOSYNTHETIC MEMBRANES
RADIOISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES