Partial purification of a monocyte-derived neutrophil chemotactic factor
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6906960
The movement of neutrophils (PMN's) into a wound site is believed to be regulated by the presence of various chemoattractants secreted during the wound repair process. They have now identified and partially characterized a new PMN chemoattractant obtained from human peripheral blood monocyte conditioned medium. The conditioned medium was tested for PMN chemotactic activity using Boyden chambers containing /sup 51/Cr-labeled human PMN's. A biphasic dose response curve with maximal activity at 8 ..mu..g/ml was obtained from concentrated serum-free conditioned medium. Fast protein liquid chromatography (FPLC) using a Mono Q ion exchange resin revealed that the chemotactic activity could be eluted as a single peak between 0.13 and 0.14M NaCl. These active fractions were then pooled and chromatographed on a Superose-12 column. A single peak of activity was recovered which corresponded to a molecular weight of 3-5000 da. Checkerboard analysis indicated that the activity was indeed due to chemotaxis rather than chemokinesis. These results suggest that monocytes are capable of secreting a powerful chemoattractant for PMN's.
- Research Organization:
- Univ. of Texas Medical Branch and Shriners Burns Institute, Galveston
- OSTI ID:
- 6906960
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Alveolar macrophage-stimulated neutrophil and monocyte migration: effects of in vitro ozone exposure
Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines
Characterization of a receptor for human monocyte-derived neutrophil chemotactic factor/interleukin-8
Journal Article
·
Thu Mar 31 23:00:00 EST 1988
· Toxicol. Appl. Pharmacol.; (United States)
·
OSTI ID:6991702
Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines
Journal Article
·
Mon Nov 30 23:00:00 EST 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6326010
Characterization of a receptor for human monocyte-derived neutrophil chemotactic factor/interleukin-8
Journal Article
·
Tue May 15 00:00:00 EDT 1990
· Journal of Biological Chemistry; (USA)
·
OSTI ID:7045402
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMATOGRAPHY
CHROMIUM 51
CHROMIUM ISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEALING
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
MOLECULAR WEIGHT
MONOCYTES
NEUTROPHILS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
PURIFICATION
RADIOISOTOPES
RECOVERY
SEPARATION PROCESSES
TRACER TECHNIQUES
WOUNDS
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMATOGRAPHY
CHROMIUM 51
CHROMIUM ISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEALING
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
MOLECULAR WEIGHT
MONOCYTES
NEUTROPHILS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
PURIFICATION
RADIOISOTOPES
RECOVERY
SEPARATION PROCESSES
TRACER TECHNIQUES
WOUNDS