Proteins induced by salt stress in tomato germinating seeds
- Instituto Recursos Naturales y Agrobiologia, Sevilla (Spain)
Salt effects on protein synthesis in tomato germinating seeds were investigated by two-dimensional polyacrilamide gel electrophoresis of proteins labeled in vivo with ({sup 35}S)-Methionine. Seeds germinating in NaCl were analyzed at three germination stages (4mm long radicals, 15mm long radicles and expanding cotyledons) and compared to those germinating in water. At the first germination stage several basic proteins of M.W. 13Kd, 16Kd, 17Kd and 18Kd were detected in only salt germinating seeds. Other basic proteins of M.W. 12Kd, 50Kd and 54Kd were salt-induced at the second and third stage of germination. One 14Kd acid protein is observed in every assayed stage and shows several phosphorylated forms. The levels of expression of these proteins are directly correlated to assayed NaCl concentrations. All of these proteins, except 17Kd, are also induced by abscisic acid (ABA) in the same germination stages. A cooperative effect on the synthesis of these proteins is observed when both ABA and NaCl are present.
- OSTI ID:
- 6906491
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
BIOSYNTHESIS
SODIUM CHLORIDES
BIOLOGICAL EFFECTS
ABSCISIC ACID
GERMINATION
METHIONINE
PLANTS
SEEDS
SULFUR 35
TOMATOES
TRACER TECHNIQUES
ALKALI METAL COMPOUNDS
AMINO ACIDS
AUXINS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FOOD
FRUITS
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANT GROWTH REGULATORS
RADIOISOTOPES
SODIUM COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques