Characterization of elemental sulfur in isolated intact spinach chloroplasts. [Spinacia oleracea L]

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.88.4.961· OSTI ID:6906198

Joyard, J; Douce, R ^[1]; Forest, E ^[2]; Blee, E ^[3]

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1. Laboratoire Mixte CNRS/Rhone-Poulenc, Lyon (France)
2. Centre d'Etudes Nucleaires de Grenoble (France)
3. Institut de Botanique, Strasbourg (France)

Incubation of intact spinach (Spinacia oleracea L.) chloroplasts in the presence of {sup 35}SO{sub 4}{sup 2{minus}} resulted in the light-dependent formation of a chloroform-soluble sulfur-containing compound distinct from sulfolipid. The authors have identified this compound as the most stable form (S{sub 8}) of elemental sulfur (S{sup 0}, valence state for S = O) by mass spectrometry. It is possible that elemental sulfur (S{sup 0}) was formed by oxidation of bound sulfide, i.e. after the photoreduction of sulfate to sulfide by intact chloroplasts, and released as S{sub 8} under the experimental conditions used for analysis.

• OSTI ID: 6906198
• Journal Information: Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 88:4; ISSN PLPHA; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CELL CONSTITUENTS
CHEMICAL COMPOSITION
CHLOROPLASTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
FOOD
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MASS SPECTROSCOPY
NONMETALS
NUCLEI
OXYGEN COMPOUNDS
PHYSIOLOGY
PLANTS
RADIOISOTOPES
SPECTROSCOPY
SPINACH
SULFATES
SULFUR
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VEGETABLES