Pathways of nitrogen assimilation in cowpea nodules studied using /sup 15/N/sub 2/ and allopurinol. [Vigna unguiculata L. Walp. cv Vita]
Journal Article
·
· Plant Physiol.; (United States)
OSTI ID:6905633
In the presence of 0.5 millimolar allopurinol (4-hydroxypyrazolo (3,4-d)pyrimidine), an inhibitor of NAD:xanthine oxidoreductase (EC 1.2.3.2), intact attached nodules of cowpea (vigna unguiculata L. Walp. cv Vita 3) formed (/sup 15/N)xanthine from /sup 15/N/sub 2/ at rates equivalent to those of ureide synthesis, confirming the direct assimilation of fixed nitrogen into purines. Xanthine accumulated in nodules and was exported in increasing amounts in xylem of allopurinol-treated plants. Other intermediates of purine oxidation, de novo purine synthesis, and ammonia assimilation did not increase and, over the time course of experiments (4 hours), allopurinol had no effect on nitrogenase (EC 1.87.99.2) activity. Negligible /sup 15/N -labeling of asparagine from /sup 15/N/sub 2/ was observed, suggesting that the significant pool (up to 14 micromoles per gram of nodule fresh weight) of this amide in cowpea nodules was not formed directly from fixation but may have accumulated as a consequence of phloem delivery.
- Research Organization:
- Univ. of Western Australia, Nedlands
- OSTI ID:
- 6905633
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 86:1; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
AMMONIA
AROMATICS
ASPARAGINE
AZAARENES
BIOLOGICAL ACCUMULATION
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACIDS
ELEMENTS
ENZYMES
FOOD
HETEROCYCLIC COMPOUNDS
HYDRIDES
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NITRO-GROUP DEHYDROGENASES
NITROGEN
NITROGEN 15
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NITROGENASE
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
PEAS
PLANTS
PURINES
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VEGETABLES
XANTHINES
59 BASIC BIOLOGICAL SCIENCES
AMIDES
AMINO ACIDS
AMMONIA
AROMATICS
ASPARAGINE
AZAARENES
BIOLOGICAL ACCUMULATION
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOXYLIC ACIDS
ELEMENTS
ENZYMES
FOOD
HETEROCYCLIC COMPOUNDS
HYDRIDES
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NITRO-GROUP DEHYDROGENASES
NITROGEN
NITROGEN 15
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NITROGENASE
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
PEAS
PLANTS
PURINES
STABLE ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
VEGETABLES
XANTHINES