Characterization of D-enzyme (4-. cap alpha. -glucanotransferase) in Arabidopsis leaf

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Plant Physiol.; (United States)

OSTI ID:6905618

Lin, T P; Preiss, J

Two major forms of D-enzyme (4-..cap alpha..-glucanotransferase, EC 2.4.1.25) were successfully separated from most of the amylase activity using FPLC-Mono Q column chromatography. Transfer of a maltosyl group was observed upon the incubation of D-enzyme with maltotriose and D-(U-/sup 14/C)glucose. About 4.5% of the radioactivity was transferred to maltotriose in 2 hours. End product analysis showed the accumulation of glucose and maltopentaose from maltotriose within the first 10 minutes of the reaction. Several other maltodextrins were also observed with longer incubation times, although maltose was never produced. A quantitative measurement of maltodextrin production from the reaction of (/sup 14/C)maltotriose with D-enzyme showed that the quantity of maltotriose decreased from 100% to 31% after 3 hours incubation, while glucose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, maltooctaose, and higher maltodextrins increased in amount. Glucose is the major product throughout the course of the reaction of D-enzyme with maltotriose. Maltotriose, in addition to glucose, are the major products in the reaction of D-enzyme with maltodextrins with a chain length greater than maltotriose. This study confirms the existence of a transglycosylase that disproportionates amaltotriose and higher maltodextrins by transferring maltosyl or maltodextrinyl groups between maltodextrins resulting in the production of glucose and different maltodextrins, but not maltose.

• Research Organization: Michigan State Univ., East Lansing (USA)
• OSTI ID: 6905618
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 86:1; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DISACCHARIDES
ENZYME ACTIVITY
ENZYMES
GLUCOSE
HEXOSES
INCUBATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAVES
LIQUID COLUMN CHROMATOGRAPHY
MALTOSE
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRANSFERASES