Effects of brussels sprouts, indole 3-carbinol and phenobarbital on xenobiotic metabolism and in vivo DNA binding of aflatoxin B/sub 1/ in the rat
Cruciferous vegetables have been shown to be potent inducers of xenobiotic-metabolizing enzymes in the rat and this may offer protection against chemical carcinogenesis. Adult, male, SD rats were fed on purified diets supplemented with 25% freeze-dried Brussels sprouts or 250 ppm idole 3-carbinol (I3C) for 2 weeks, or given phenobarbital (PB, 1 mg/ml) in the drinking water for 7 days prior to killing. Brussels sprouts caused a 50% decrease (p < 0.001) in the in vivo binding of (/sup 3/H) aflatoxin B/sub 1/ (AFB/sub 1/) to liver DNA, and increased intestinal and hepatic glutathione S-transferase (GST) activity. Hepatic monooxygenase activity was not altered in this group but greater than 2-fold increases in intestinal aryl hydrocarbon hydroxylase (AHH) and ethoxycoumarin O-deethylase (ECD) activities were found. I3C did not decrease AFB/sub 1/ binding, nor did it increase hepatic or intestinal GST activity. I3C did increase both intestinal AHH and ECD activities. PB treatment significantly decreased AFB/sub 1/ binding by 60%, and significantly elevated hepatic but not intestinal GST activity. Hepatic AHH and ECD activities were also elevated in this group, while intestinal AHH and ECD activities were decreased. These results emphasize the importance of GST activity in the detoxification of AFB/sub 1/ and suggest a less important role for intestinal monooxygenase activity in the metabolism of this hepatocarcinogen.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 6904912
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANESTHETICS
ANIMALS
ANTICONVULSANTS
ANTIGENS
AZINES
BARBITURATES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BRASSICA
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DNA
DRUGS
ENZYME ACTIVITY
ENZYMES
FOOD
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXYLASES
HYPNOTICS AND SEDATIVES
IN VIVO
KINETICS
LIVER
MAMMALS
MATERIALS
METABOLISM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PHENOBARBITAL
PLANTS
PYRIMIDINES
RATS
REACTION KINETICS
RODENTS
TOXIC MATERIALS
TOXINS
TRANSFERASES
VEGETABLES
VERTEBRATES
XENOBIOTICS