Chronic ethanol administration increases the binding of sup 3 H Ro-15-4513 in primary cultured spinal cord neurons
Journal Article
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (USA)
OSTI ID:6904903
- Univ. of Texas Health Science Center, San Antonio (USA)
Ro 15-4513 (ethyl-8-azido-5, 6-dihydro-5-methyl-6-oxo-4H-imidazo (1,5{alpha}), (1,4) benzodiazepine-3-carboxylate) is reported to be a selective ethanol antagonist in biochemical and behavioral studies. The effect of chronic ethanol treatment on the binding of ({sup 3}H)Ro 15-4513 was investigated in cultured spinal cord neurons, which are shown to possess all the elements of GABA benzodiazepine receptor complex. Chronic ethanol treatment (50 mM for 6 hr, 12 hr, 18 hr, 3 days, and 5{sub 3} days) produced an increase in the specific binding of ({sub 3}H)Ro 15-4513. The increase in binding in these neurons was due to an increase in the number (B{sub max}) of receptor sites. This effect was specific for Ro 15-4513, since identical ethanol treatment did not alter the binding of benzodiazepine antagonist ({sup 3}H)Ro 15-1788 or agonist ({sup 3}H)flunitrazepam or inverse agonist ({sup 3}H)methyl-{beta}-carboline-3-carboxylate. Similar results have been reported following chronic ethanol treatment to rats. These results suggest that the Ro 15-4513 binding sites on the oligomeric GABA receptor complex are altered following chronic ethanol administration, and support the notion of a unique role of Ro 15-4513 as an ethanol antagonist.
- OSTI ID:
- 6904903
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (USA) Vol. 3:3; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Journal Article
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Mon Aug 01 00:00:00 EDT 1988
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OSTI ID:7036815
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Tue May 01 00:00:00 EDT 1990
· Journal of Pharmacology and Experimental Therapeutics; (USA)
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OSTI ID:6721290
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Journal Article
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Thu Feb 28 23:00:00 EST 1991
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·
OSTI ID:5549119
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
CHRONIC INTAKE
DRUGS
ETHANOL
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INTAKE
ISOTOPE APPLICATIONS
KINETICS
NERVE CELLS
ORGANIC COMPOUNDS
REACTION KINETICS
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
CHRONIC INTAKE
DRUGS
ETHANOL
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INTAKE
ISOTOPE APPLICATIONS
KINETICS
NERVE CELLS
ORGANIC COMPOUNDS
REACTION KINETICS
SOMATIC CELLS
TOXICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS