Structural organization of the porcine and human genes coding for a leydig cell-specific insulin-like peptide (LEY I-L) and chromosomal localization of the human gene (INSL3)

Journal Article · Tue Mar 01 04:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1121· OSTI ID:6904030

Burkhardt, E; Adham, I M; Brosig, B; Gastmann, A; Engel, W ^[1]; Mattei, M G ^[2]

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1. Institut fuer Humangenetik der Universitaet, Goettingen (Germany)
2. INSERM, Marseille (France)

Leydig insulin-like protein (LEY I-L) is a member of the insulin-like hormone superfamily. The LEY I-L gene (designated INSL3) is expressed exclusively in prenatal and postnatal Leydig cells. The authors report here the cloning and nucleotide sequence of porcine and human LEY I-L genes including the 5[prime] regions. Both genes consist of two exons and one intron. The organization of the LEY I-L gene is similar to that of insulin and relaxin. The transcription start site in the porcine and human LEY I-L gene is localized 13 and 14 bp upstream of the translation start site, respectively. Alignment of the 5[prime] flanking regions of both genes reveals that the first 107 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 80%. This conserved region contains a consensus TATAA box, a CAAT-like element (GAAT), and a consensus SP1 sequence (GGGCGG) at equivalent positions in both genes and therefore may play a role in regulation of expression of the LEY I-L gene. The porcine and human genome contains a single copy of the LEY I-L gene. By in situ hybridization, the human gene was assigned to bands p13.2-p12 of the short arm of chromosome 19. 25 refs., 6 figs.

• OSTI ID: 6904030
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 20:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BODY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
GONADS
HORMONES
HUMAN CHROMOSOME 19
HUMAN CHROMOSOMES
HYBRIDIZATION
MALE GENITALS
MAPPING
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TESTES