Transcriptional regulation of coordinate changes in flagellar mRNAs during differentiation of Naegleria gruberi amoebae into flagellates

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.6.2280· OSTI ID:6902979

Lee, J H; Walsh, C J

The nuclear run-on technique was used to measure the rate of transcription of flagellar genes during the differentiation of Naegleria gruberi amebae into flagellates. Synthesis of mRNAs for the axonemal proteins ..cap alpha..- and BETA-tubulin and flagellar calmodulin, as well as a coordinately regulated poly(A)/sup +/ RNA that codes for an unidentified protein, showed transient increases averaging 22-fold. The rate of synthesis of two poly(A)/sup +/ RNAs common to ameobae and flagellates was low until the transcription of the flagellar genes began to decline, at which time synthesis of the RNAs found in ameobae increased 3- to 10-fold. The observed changes in the rate of transcription can account quantitatively for the 20-fold increase in flagellar mRNA concentration during the differentiation. The data for the flagellar calmodulin gene demonstrate transcriptional regulation for a nontubulin axonemal protein. The data also demonstrate at least two programs of transcriptional regulation during the differentiation and raise the intriguing possibility that some significant fraction of the nearly 200 different proteins of the flagellar axoneme is transcriptionally regulated during the 1 h it takes N. gruberi amebae to form visible flagella.

• Research Organization: Dept. of Biological Sciences, Univ. of Pittsburgh, Pittsburgh, PA (US)
• OSTI ID: 6902979
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALMODULIN
GENE REGULATION
PROTAMINES
CELL DIFFERENTIATION
TRANSCRIPTION
MOLECULAR BIOLOGY
TUBULES
AMOEBA
GENE REPRESSORS
MESSENGER-RNA
ANIMALS
BODY
COAGULANTS
DRUGS
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
INVERTEBRATES
KIDNEYS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
PROTOZOA
RNA
SARCODINA
550200* - Biochemistry
550800 - Morphology