Comparative studies of nucleic acid hybridization assay for Listeria in foods
A nucleic acid hybridization assay has been developed for Listeria spp. in dairy foods and environmental samples. The assay is based on detection of unique Listeria 16S rRNA sequences by using a /sup 32/P-labeled synthetic DNA probe. Inclusivity and exclusivity of the probe were confirmed with 139 Listeria isolates representing all known species, and 73 non-Listeria bacterial strains. In this paper, we present results from our preliminary studies comparing the hybridization assay with conventional culture on a total of 575 specimens that represent a variety of inoculated and uninoculated foods and environmental samples. The assay, which is done in a filter manifold format after 2 days of cultural enrichment, requires a total assay time of less than 2.5 days. The false-negative rate for all sample groups tested using the GENE-TRAK hybridization assay was less than the rate for culture. Thus, the new assay allows rapid screening of the indicated product groups and provides reliable numerical results.
- Research Organization:
- GENE-TRAK Systems, Framingham, MA (USA)
- OSTI ID:
- 6902180
- Journal Information:
- J. Assoc. Off. Anal. Chem.; (United States), Vol. 71:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PATHOGENS
RADIOASSAY
ACCURACY
COMPARATIVE EVALUATIONS
DNA
ENVIRONMENTAL MATERIALS
HYBRIDIZATION
MILK
MILK PRODUCTS
PHOSPHORUS 32
RNA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
FOOD
ISOTOPES
LIGHT NUCLEI
MATERIALS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)